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(E. coli and Salmonella) isolated from the fruit area wash as well as the juice demonstrate that E. coli is uncovered on fruit (3%) and from the juice (2%). Salmonella is infrequently uncovered, but our knowledge exhibit that it are available in the juice when it can be on the outside on the fruit. At the moment, 25 samples Each and every from the 3 groups of fruit described (unwashed and ungraded, washed and graded and cull fruit) are utilized. Additional alterations were being manufactured within the wash technique and during the preliminary isolation of pathogens.

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A small amount of buffer extra to every sample fruit inside the sterile whirlpak bag was substituted for your sterile sponge process described inside the exploration proposal. A part of the buffer sample from Every single washed fruit was placed in pre-enrichment broth For additional productive isolation of any pathogens (e.g. E. coli and Salmonella) That may be present in compact amounts. Studies around the fruit interiors (juice) have been changed marginally to allow for better processing of samples. Fruit is sanitized

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